Optimization of KNO3 Concentration in Breaking Dormancy and Enhancing Seed Vigor of Hevea brasiliensis

Authors

DOI:

https://doi.org/10.56207/genbionix.v4i1.850

Keywords:

seed dormancy, potassium nitrate, seed vigor, Hevea brasiliensis

Abstract

Seed dormancy remains one of the major constraints affecting the propagation and seedling availability of Hevea brasiliensis in rubber cultivation systems. Low germination percentage and poor seed vigor often reduce seedling quality and hinder plantation productivity. This study aimed to determine the optimal concentration of Potassium Nitrate (KNO3) in breaking seed dormancy and enhancing seed vigor of Hevea brasiliensis. The study was conducted using a Completely Randomized Design (CRD) consisting of five treatments, namely 0%, 10%, 20%, 30%, and 40% KNO3 concentrations with four replications. Rubber seeds were soaked in KNO3 solutions for 72 hours and observed for 60 days. Parameters measured included germination percentage, germination rate, plumule length, and root length. Data were analyzed using One-Way Analysis of Variance (ANOVA) followed by Least Significant Difference (LSD) and Duncan’s Multiple Range Test (DMRT) at a 5% significance level. The results showed that KNO3 treatment significantly affected dormancy breaking and seed vigor enhancement. The 40% KNO3 treatment produced the highest germination percentage, fastest germination rate, and best seedling growth performance compared to other treatments. These findings indicated that KNO3 application effectively stimulated germination activity and improved the physiological quality of rubber seeds.

References

Ahmad, S., Wang, G.-Y., Muhammad, I., Zeeshan, M., & Zhou, X.-B. (2022). Melatonin and KNO3 Application Improves Growth, Physiological and Biochemical Characteristics of Maize Seedlings under Waterlogging Stress Conditions. Biology, 11(1), 99. https://doi.org/10.3390/biology11010099

Ahmadi, K., Omidi, H., & Soltani, E. (2023). Efficacy of KNO3 and priming duration for improving germination, seedling growth, and antioxidant enzymes of Keluss (Kelussia odoratissma Mozaff). Acta Ecologica Sinica, 43(6), 1120–1128. https://doi.org/10.1016/j.chnaes.2023.04.003

Ali, A. (2020). Nitrate assimilation pathway in higher plants: critical role in nitrogen signalling and utilization. Plant Science Today, 7(2), 182–192. https://doi.org/10.14719/pst.2020.7.2.637

Ali, L. G., Nulit, R., Ibrahim, M. H., & Yien, C. Y. S. (2021a). Efficacy of KNO3, SiO2 and SA priming for improving emergence, seedling growth and antioxidant enzymes of rice (Oryza sativa), under drought. Scientific Reports, 11(1), 3864. https://doi.org/10.1038/s41598-021-83434-3

Ali, L. G., Nulit, R., Ibrahim, M. H., & Yien, C. Y. S. (2021b). Potassium nitrate and silicon dioxide priming improve germination, seedling growth and protective enzymes of rice var. FARO44 under drought. Journal of Plant Nutrition, 44(16), 2385–2398. https://doi.org/10.1080/01904167.2021.1921201

Bhatla, S. C., & Kathpalia, R. (2023). Seed Dormancy and Germination. In Plant Physiology, Development and Metabolism (pp. 625–640). Springer Nature Singapore. https://doi.org/10.1007/978-981-99-5736-1_28

Da Rocha, L. G., Rau, B. A., Da Silva, D. M., De Araújo, R. M., & Masetto, T. E. (2025). Potassium nitrate (kno₃) seed priming enhances soybean seed performance. Scientific Reports, 15(1), 33640. https://doi.org/10.1038/s41598-025-11498-6

de Carvalho, J. C., Gonçalves, K. D., Gonçalves, E. V., dos Santos, P. P., Miranda, M. de J. de S., Nina Junior, A. da R., Nunes-Nesi, A., Araújo, W. L., & Gonçalves, J. F. de C. (2023). Morphophysiological indicators applied for the selection of different genotypes of Hevea spp. during germination and post-germination. Botany, 101(10), 449–461. https://doi.org/10.1139/cjb-2023-0020

Javed, T., Ali, M. M., Shabbir, R., Gull, S., Ali, A., Khalid, E., Abbas, A. N., Tariq, M., & Muqmirah. (2020). Rice Seedling Establishment as Influenced by Cultivars and Seed Priming With Potassium Nitrate. Journal of Applied Research in Plant Sciences, 1(2), 65–75. https://doi.org/10.38211/joarps.2020.1.2.10

Kurnia, D., Marimin, Haris, U., & Sudradjat. (2020). Critical issue mapping of Indonesian natural rubber industry based on innovation system perspectives. IOP Conference Series: Earth and Environmental Science, 443(1), 012036. https://doi.org/10.1088/1755-1315/443/1/012036

Narejo, G. A., Mirbahar, A. A., Yasin, S., Sirohi, M. H., & Saeed, R. (2023). Effect of Hydro and KNO3 Priming on Seed Germination of Cotton (Gossypium hirsutum L.) Under Gnotobiotic Conditions. Journal of Plant Growth Regulation, 42(3), 1592–1603. https://doi.org/10.1007/s00344-022-10644-y

Nautiyal, P. C., Sivasubramaniam, K., & Dadlani, M. (2023). Seed Dormancy and Regulation of Germination. In Seed Science and Technology (pp. 39–66). Springer Nature Singapore. https://doi.org/10.1007/978-981-19-5888-5_3

Nurul Asyiqin, A. B., & Ong, K. H. (2025). Adapting forest plantations to climate change: strategies, challenges, and opportunities in Malaysia. New Forests, 56(3), 35. https://doi.org/10.1007/s11056-025-10105-w

Pompelli, M. F., Jarma-Orozco, A., & Rodriguez-Páez, L. A. (2023). Imbibition and Germination of Seeds with Economic and Ecological Interest: Physical and Biochemical Factors Involved. Sustainability, 15(6), 5394. https://doi.org/10.3390/su15065394

Reed, R. C., Bradford, K. J., & Khanday, I. (2022). Seed germination and vigor: ensuring crop sustainability in a changing climate. Heredity, 128(6), 450–459. https://doi.org/10.1038/s41437-022-00497-2

Sari, I. L., Weston, C. J., Newnham, G. J., & Volkova, L. (2021). Developing Multi-Source Indices to Discriminate between Native Tropical Forests, Oil Palm and Rubber Plantations in Indonesia. Remote Sensing, 14(1), 3. https://doi.org/10.3390/rs14010003

Zebua, N., Habibah, L. B., & Zebua, E. N. K. (2026). Problems of Forest and Biodiversity Conservation in Developing Countries. In Protecting Biodiversity, Culture, and Communities Through Forest Conservation (pp. 171–198). IGI Global Scientific Publishing. https://doi.org/10.4018/979-8-3373-3992-4.ch006

Ziraluo, Y. P. B., & Duha, M. (2020). Diversity study of fruit producer plant in Nias Islands. Jurnal Inovasi Penelitian, 1(4), 683–694.

Ziraluo, Y. P. B., & Zebua, N. (2026). Ecological Literacy Based on Local Wisdom of the Nias Community as a Framework for Sustainable Environmental Education. Jurnal Pendidikan Biologi Undiksha, 13(1), 113–122. https://doi.org/10.23887/jjpb.v13i1.110656

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Published

29-05-2026

How to Cite

Ziraluo, Y. P. B. (2026). Optimization of KNO3 Concentration in Breaking Dormancy and Enhancing Seed Vigor of Hevea brasiliensis. GEN BIONIX: Jurnal Ilmiah Pendidikan Biologi, 4(1), 34–39. https://doi.org/10.56207/genbionix.v4i1.850