Optimization of KNO3 Concentration in Breaking Dormancy and Enhancing Seed Vigor of Hevea brasiliensis
DOI:
https://doi.org/10.56207/genbionix.v4i1.850Keywords:
seed dormancy, potassium nitrate, seed vigor, Hevea brasiliensisAbstract
Seed dormancy remains one of the major constraints affecting the propagation and seedling availability of Hevea brasiliensis in rubber cultivation systems. Low germination percentage and poor seed vigor often reduce seedling quality and hinder plantation productivity. This study aimed to determine the optimal concentration of Potassium Nitrate (KNO3) in breaking seed dormancy and enhancing seed vigor of Hevea brasiliensis. The study was conducted using a Completely Randomized Design (CRD) consisting of five treatments, namely 0%, 10%, 20%, 30%, and 40% KNO3 concentrations with four replications. Rubber seeds were soaked in KNO3 solutions for 72 hours and observed for 60 days. Parameters measured included germination percentage, germination rate, plumule length, and root length. Data were analyzed using One-Way Analysis of Variance (ANOVA) followed by Least Significant Difference (LSD) and Duncan’s Multiple Range Test (DMRT) at a 5% significance level. The results showed that KNO3 treatment significantly affected dormancy breaking and seed vigor enhancement. The 40% KNO3 treatment produced the highest germination percentage, fastest germination rate, and best seedling growth performance compared to other treatments. These findings indicated that KNO3 application effectively stimulated germination activity and improved the physiological quality of rubber seeds.
References
Ahmad, S., Wang, G.-Y., Muhammad, I., Zeeshan, M., & Zhou, X.-B. (2022). Melatonin and KNO3 Application Improves Growth, Physiological and Biochemical Characteristics of Maize Seedlings under Waterlogging Stress Conditions. Biology, 11(1), 99. https://doi.org/10.3390/biology11010099
Ahmadi, K., Omidi, H., & Soltani, E. (2023). Efficacy of KNO3 and priming duration for improving germination, seedling growth, and antioxidant enzymes of Keluss (Kelussia odoratissma Mozaff). Acta Ecologica Sinica, 43(6), 1120–1128. https://doi.org/10.1016/j.chnaes.2023.04.003
Ali, A. (2020). Nitrate assimilation pathway in higher plants: critical role in nitrogen signalling and utilization. Plant Science Today, 7(2), 182–192. https://doi.org/10.14719/pst.2020.7.2.637
Ali, L. G., Nulit, R., Ibrahim, M. H., & Yien, C. Y. S. (2021a). Efficacy of KNO3, SiO2 and SA priming for improving emergence, seedling growth and antioxidant enzymes of rice (Oryza sativa), under drought. Scientific Reports, 11(1), 3864. https://doi.org/10.1038/s41598-021-83434-3
Ali, L. G., Nulit, R., Ibrahim, M. H., & Yien, C. Y. S. (2021b). Potassium nitrate and silicon dioxide priming improve germination, seedling growth and protective enzymes of rice var. FARO44 under drought. Journal of Plant Nutrition, 44(16), 2385–2398. https://doi.org/10.1080/01904167.2021.1921201
Bhatla, S. C., & Kathpalia, R. (2023). Seed Dormancy and Germination. In Plant Physiology, Development and Metabolism (pp. 625–640). Springer Nature Singapore. https://doi.org/10.1007/978-981-99-5736-1_28
Da Rocha, L. G., Rau, B. A., Da Silva, D. M., De Araújo, R. M., & Masetto, T. E. (2025). Potassium nitrate (kno₃) seed priming enhances soybean seed performance. Scientific Reports, 15(1), 33640. https://doi.org/10.1038/s41598-025-11498-6
de Carvalho, J. C., Gonçalves, K. D., Gonçalves, E. V., dos Santos, P. P., Miranda, M. de J. de S., Nina Junior, A. da R., Nunes-Nesi, A., Araújo, W. L., & Gonçalves, J. F. de C. (2023). Morphophysiological indicators applied for the selection of different genotypes of Hevea spp. during germination and post-germination. Botany, 101(10), 449–461. https://doi.org/10.1139/cjb-2023-0020
Javed, T., Ali, M. M., Shabbir, R., Gull, S., Ali, A., Khalid, E., Abbas, A. N., Tariq, M., & Muqmirah. (2020). Rice Seedling Establishment as Influenced by Cultivars and Seed Priming With Potassium Nitrate. Journal of Applied Research in Plant Sciences, 1(2), 65–75. https://doi.org/10.38211/joarps.2020.1.2.10
Kurnia, D., Marimin, Haris, U., & Sudradjat. (2020). Critical issue mapping of Indonesian natural rubber industry based on innovation system perspectives. IOP Conference Series: Earth and Environmental Science, 443(1), 012036. https://doi.org/10.1088/1755-1315/443/1/012036
Narejo, G. A., Mirbahar, A. A., Yasin, S., Sirohi, M. H., & Saeed, R. (2023). Effect of Hydro and KNO3 Priming on Seed Germination of Cotton (Gossypium hirsutum L.) Under Gnotobiotic Conditions. Journal of Plant Growth Regulation, 42(3), 1592–1603. https://doi.org/10.1007/s00344-022-10644-y
Nautiyal, P. C., Sivasubramaniam, K., & Dadlani, M. (2023). Seed Dormancy and Regulation of Germination. In Seed Science and Technology (pp. 39–66). Springer Nature Singapore. https://doi.org/10.1007/978-981-19-5888-5_3
Nurul Asyiqin, A. B., & Ong, K. H. (2025). Adapting forest plantations to climate change: strategies, challenges, and opportunities in Malaysia. New Forests, 56(3), 35. https://doi.org/10.1007/s11056-025-10105-w
Pompelli, M. F., Jarma-Orozco, A., & Rodriguez-Páez, L. A. (2023). Imbibition and Germination of Seeds with Economic and Ecological Interest: Physical and Biochemical Factors Involved. Sustainability, 15(6), 5394. https://doi.org/10.3390/su15065394
Reed, R. C., Bradford, K. J., & Khanday, I. (2022). Seed germination and vigor: ensuring crop sustainability in a changing climate. Heredity, 128(6), 450–459. https://doi.org/10.1038/s41437-022-00497-2
Sari, I. L., Weston, C. J., Newnham, G. J., & Volkova, L. (2021). Developing Multi-Source Indices to Discriminate between Native Tropical Forests, Oil Palm and Rubber Plantations in Indonesia. Remote Sensing, 14(1), 3. https://doi.org/10.3390/rs14010003
Zebua, N., Habibah, L. B., & Zebua, E. N. K. (2026). Problems of Forest and Biodiversity Conservation in Developing Countries. In Protecting Biodiversity, Culture, and Communities Through Forest Conservation (pp. 171–198). IGI Global Scientific Publishing. https://doi.org/10.4018/979-8-3373-3992-4.ch006
Ziraluo, Y. P. B., & Duha, M. (2020). Diversity study of fruit producer plant in Nias Islands. Jurnal Inovasi Penelitian, 1(4), 683–694.
Ziraluo, Y. P. B., & Zebua, N. (2026). Ecological Literacy Based on Local Wisdom of the Nias Community as a Framework for Sustainable Environmental Education. Jurnal Pendidikan Biologi Undiksha, 13(1), 113–122. https://doi.org/10.23887/jjpb.v13i1.110656
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Yan Piter Basman Ziraluo, Published by Department of Biology Education, Faculty of Teacher Tr

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.









